Lasers for Neuroscience
Easy to use fiber laser for 2-Photon Microscopy and 2-Photon Optogenetics in NeuroscienceGräfelfing, )
To drive this nonlinear process and to resolve the neurons deep within the living brain, femtosecond lasers with clean temporal pulse shape and average output powers of >1W are an essential prerequisite.
Going beyond pure imaging, all-optical interrogation is a novel approach to understand how active patterns in neuronal activity drive behavior. In such experiments the visualization of neuronal activity by 2-photon imaging is combined with 2-photon optogenetics to stimulate individual neurons by photoactivation of channelrhodopsins within the cell.
With the need of exciting many neurons in parallel, the laser requirements and microscopy technology for 2-photon optogenetics are fundamentally different from 2-photon imaging. Typically, high-power multi-Watt lasers at 1030-1040nm with repetition rates in the 100kHz - 1MHz range are used in combination with a spatial light modulator (SLM) to excite 10s to 100s of neurons simultaneously.
To support and drive the research in neuroscience TOPTICA is proud to introduce our laser portfolio for 2-photon imaging of GFPs and RFPs, our FemtoFiber ultra 920 and FemtoFiber ultra 1050, and for 2-photon optogenetics of channelrhodopsins our FemtoFiber vario 1030 HP:
For details and help please get in touch: www.toptica.com
All lasers are fully matched to the requirements in neuroscientific research and besides being fully turn-key, quite, and compact, they are offering integrated dispersion pre-compensation (GDD) and integrated power control (AOM) to simplify operation and to allow the scientist to focus on their research!