SIRION Biotech presents its new line of products under the new SenCELL(TM) brand for functionality testing in stable in vitro cell systems.
An example is SenCELL LifeActTM representing a range of First in Class Sensor Cell Lines with sensor molecules that identify actin dynamics and at the same time show no interference with dynamic processes during cytoskeleton rearrangements. In detail, SenCELL LifeActTM express a small actin binding protein domain coupled to a fluorescent protein.
This way, live cell imaging and high content analysis are now applicable to a wide variety of assays including migration assays using chemoattractants. The cell lines are functionally validated, available in bulk and shipped in frozen cell stocks. Applicable in a matter of days.
Initial ready-to-use SenCELL LifeActTM are already commercially available: LifeAct HT1080 and soon LifeAct MDCK . The picture is showing a A549 cell, transduced with rAVCMV-LifeAct-TagGFP2 (green) - courtesy of ibidi - cells in focus .
Another even quite common example is SenCELL HypoxiaTM - a reporter cell line system for in vitro imaging of hypoxia: hypoxic microenvironment is coincidental with the development and therapy resistance of tumors, hence, studying hypoxia remains a focus in oncology research. SenCELL Hypoxia is taking advantage of the selective induction of the hypoxia induced transcription factor HIF1A.
A luciferase reporter gene is fused to the oxygen dependent degradation (ODD) domain of HIF1A (ODD-Luc2). Thus, under normoxic conditions luciferase is constantly posttranscriptionally modified followed by proteasomal degradation. Short half-life reporter protein of just 6 minutes gives the researcher fast & dynamic readout. It also provides a rapid response to hypoxia mimetics, proteasome inhibitors and prolyl hydroxylase inhibitors.
Example: generation of an ODD-Luciferase colon cancer reporter cell line by lentiviral transduction; hypoxia induction using cobalt chloride, result: Chemoluminescence is highly induced after CoCl2 treatment in ODD-Luciferase overexpressing cell line ODD-Luc2.
An example is SenCELL LifeActTM representing a range of First in Class Sensor Cell Lines with sensor molecules that identify actin dynamics and at the same time show no interference with dynamic processes during cytoskeleton rearrangements. In detail, SenCELL LifeActTM express a small actin binding protein domain coupled to a fluorescent protein.
This way, live cell imaging and high content analysis are now applicable to a wide variety of assays including migration assays using chemoattractants. The cell lines are functionally validated, available in bulk and shipped in frozen cell stocks. Applicable in a matter of days.
Initial ready-to-use SenCELL LifeActTM are already commercially available: LifeAct HT1080 and soon LifeAct MDCK . The picture is showing a A549 cell, transduced with rAVCMV-LifeAct-TagGFP2 (green) - courtesy of ibidi - cells in focus .
Another even quite common example is SenCELL HypoxiaTM - a reporter cell line system for in vitro imaging of hypoxia: hypoxic microenvironment is coincidental with the development and therapy resistance of tumors, hence, studying hypoxia remains a focus in oncology research. SenCELL Hypoxia is taking advantage of the selective induction of the hypoxia induced transcription factor HIF1A.
A luciferase reporter gene is fused to the oxygen dependent degradation (ODD) domain of HIF1A (ODD-Luc2). Thus, under normoxic conditions luciferase is constantly posttranscriptionally modified followed by proteasomal degradation. Short half-life reporter protein of just 6 minutes gives the researcher fast & dynamic readout. It also provides a rapid response to hypoxia mimetics, proteasome inhibitors and prolyl hydroxylase inhibitors.
Example: generation of an ODD-Luciferase colon cancer reporter cell line by lentiviral transduction; hypoxia induction using cobalt chloride, result: Chemoluminescence is highly induced after CoCl2 treatment in ODD-Luciferase overexpressing cell line ODD-Luc2.
