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AdenoONE(TM) makes working with adenovirus a standard procedure
The AdenoONETM Cloning Kit enables recombination based adenoviral vector generation in E.coli cells followed by production in HEK 293 cells, within less than 4 weeks. Adenovirus particles are gen-erated from an E1/E3 deleted adenoviral serotype 5 and therefore comply with required safety stand-ards. SIRION BIOTECH's patent pending BAC technology comprises 2 steps: (a) Cloning of your gene of interest (GOI) or shRNA into the pSP-Adeno shuttle plasmid and (b) FLP mediated recombi-nation in E.coli to obtain your desired Ad5 E1/E3 deleted serotype.
The AdenoONE(TM) Purification Kit enables fast and easy chromatography based adenovirus purifi-cation directly from cell pellet within just 20 minutes. 4 out of 5 purifications show significantly higher yields over current industry standards:
AdenoONE(TM) not only improves reliability of the laboratory's results, it saves time and money: the pSP-Adeno shuttle vector with in total 3 different promoters comes for € 97.5 per reaction, consuma-bles may add another € 42. Purification is just another inexpensive € 27 per reaction. Products for the time being are available via www.Sirion-Biotech.com and selected agents in Germany, Japan and USA.
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