Sandhofer Str. 116
68305 Mannheim, de
+49 (8856) 60-5252
Roche's xCELLigence Cardio Instrument Used to Detect Beating Rhythm and Proarrhythmic Effects of Compounds in Stem Cell-Derived Cardiomyocytes
In this study (1), the effects of nine compounds were tested on beating frequency (beats per minute, bpm) of hiPS and mESC cardiomyocytes. The authors reported, "The results of this initial study show that, under the right conditions, the beating frequency of a monolayer of cells can be stably recorded over several days. In addition, the xCELLigence System detects changes in beating frequency and amplitude caused by added reference compounds."
The authors conclude that xCELLigence Cardio instrument has potential for 96-well-throughput cardiotoxicity screening of the effects of compounds on rhythmic beating patterns of cardiomyocytes. They underscored the need for continuous improvements in the maturation of available cardiomyocytes and in further validation of the assay on an extended set of reference compounds with known in-vivo effects. They also indicated that the production of distinct subtypes of ventricular, atrial and nodal cardiomyocytes could open up new areas of screening for arrhythmia and cardiotoxicity.
(1) Malin K.B. Jonsson, Qing-Dong Wang, Bruno Becker: Impedance-Based Detection of Beating Rhythm and Proarrhythmic Effects of Compounds on Stem Cell-Derived Cardiomyocytes.
ASSAY and Drug Development Technologies. December 2011: 589-599.
The use of information published here for personal information and editorial processing is generally free of charge. Please clarify any copyright issues with the stated publisher before further use. In the event of publication, please send a specimen copy to firstname.lastname@example.org.