Nutlin-Induced Apoptosis Analyzed Using Roche's xCELLigence System and RealTime ready Focus Panels
In a recent study (1) Scheuermann et al. evaluated a method for analyzing nutlin 3a, a selective p53 activator, using the xCELLigence System from Roche Applied Science (SIX: RO, ROG; OTCQX: RHHBY) and Roche's LightCycler® 480 Instrument Real-Time PCR with two different RealTime ready Focus Panels.
In their study describing a model system of the cellular effects of nutlin 3a (active enantiomer) and nutlin 3b (inactive enantiomer), the authors used the xCELLigence System for continuous cell recording and the LightCycler® 480 Real-Time PCR System for qRT PCR analysis. They also performed various endpoint assays for cell viability, proliferation, and apoptosis/necrosis that provided insight into nutlin-induced cellular events. This workflow proved to be a powerful model system for cellular analysis, pinpointing the optimal time points for functional endpoint assays and qPCR. More than 50 genes were up or down regulated 6 hours after nutlin 3a treatment, with more than 140 genes in the apoptosis pathway 24 hours after nutlin 3a treatment. Both RealTime ready Focus Panels provided a comprehensive set of apoptosis and cell-cycle-related genes that corresponded specifically to nutlin 3a transcriptional activation.
SJSA1 cells treated with nutlin 3a were analyzed using four different endpoint assays for cell viability, cell proliferation, cell lysis and apoptosis/necrosis. Changes in the CI profiles could thus be attributed to a cessation in DNA synthesis 24 hours after nutlin 3a treatment. This finding is consistent with a reduction in cell viability, followed by an increase in the number of apoptotic cells and the onset of cell lysis 48 hours after nutlin 3a treatment.
The xCELLigence System monitors cellular events in real-time without the incorporation of labels. The system measures electrical impedance across interdigitated micro-electrodes integrated on the bottom of tissue culture E-Plates. The impedance measurement provides quantitative information about the biological status of the cells, including cell number, viability and morphology. A wide range of cell-based assays for both high-throughput screening and research laboratory environments can be performed on the xCELLigence System.
(1) Scheuermann et al.: Nutlin-Induced Apoptosis Analyzed using the xCELLigence System and qPCR on RealTime ready Focus Panels. Cellular Analysis Application Note No. 5 2009 (https://www.roche-applied-science.com/...)
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