Identification of Valid Endogenous Control Genes for Determining Gene Expression in Human Glioma using the Roche RealTime ready Human Reference Gene Panel
The authors found that in general glioblastoma exhibited the highest expression levels and largest variability of the candidate genes, whereas normal brain showed the lowest level. In each of the tumor subgroups and across these groups, the NormFinder analyses identified a large number of genes suitable for normalization. However, this number was drastically reduced after inclusion of normal brain into the analyses: it was expected that only GAPDH, IPO8, RPL13A, SDHA, and TBP would not be differentially expressed; statistical analysis indicated favorable stability values for all of these genes, TBP and IPO8 being the most stable. These 5 genes represent different physiological pathways and may be regarded as universal reference genes applicable for accurate normalization of gene expression in human astrocytomas of different grades (WHO Grades II-IV) alone and compared with normal brain, thereby enabling longitudinally designed studies (e.g., in astrocytoma before and after malignant transformation). The results show that the Roche RealTime ready Human Reference Gene Panel is a perfect tool for analyzing a wide variety of different genes in order to identify suitable reference genes for further relative quantification experiments.
Roche Diagnostics Deutschland GmbH
Headquartered in Basel, Switzerland, Roche is a leader in researchfocused healthcare with combined strengths in pharmaceuticals and diagnostics. Roche is the world's largest biotech company with truly differentiated medicines in oncology, virology, inflammation, metabolism and CNS. Roche is also the world leader in invitro diagnostics and tissuebased cancer diagnostics, and is a pioneer in diabetes management. Roche's personalized healthcare strategy aims at providing medicines and diagnostic tools that enable tangible improvements in the health, quality of life and survival of patients. In 2009, Roche had over 80,000 employees worldwide and invested almost 10 billion Swiss francs in R&D. The Group posted sales of 49.1 billion Swiss francs. Genentech, United States, is a wholly owned member of the Roche Group. Roche has a majority stake in Chugai Pharmaceutical, Japan. For more information: www.roche.com.
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