JPK Instruments' NanoWizard® provides valuable information about the Iceman for the Ludwig-Maximilians University in Munich and the European Academy in Bolzano
Studying historical artifacts is always of interest and when the latest nanotechnology instrumentation is applied to the analysis of ancient materials, the interest rises to a higher level. In the Department of Earth and Environmental Sciences at the Ludwig-Maximilians University in Munich, scientists work on nanoscale biomaterial characterization. Their efforts have recently been showcased in the study of mummified skin from the 5300 year old Tyrolean Iceman. By way of background, skin protects the body from pathogens and degradation. Even mummified skin retains this function. The action of microorganisms or other external influences may degrade the connective tissue and lay the subjacent tissue open. To determine the degree of tissue preservation in mummified human skin and the reason for its durability, PhDstudent Marek Janko of Professor Robert Stark's research group has investigated the structural integrity of its main protein, type I collagen using an atomic force microscope, the NanoWizard® II, from JPK Instruments.
Janko's research focuses on the study of mechanical properties of nanobio materials. Use of AFM and Raman spectroscopy are non invasive techniques and samples may be reanalyzed many times. The choice of the NanoWizard® for this work is twofold. The combination of AFM with optical microscopy enables normal histology tissue samples, 2-4 microns thick and relatively "rough" to be readily studied in ambient conditions. The optical microscope enable identification of areas for study and then the AFM is used to effectively zoom in to study individual fibrils on the tens of nanometer scale. Being able to zoom to and study a single fibril just 30-40nm in height means the individual repeat units of 67nm may be identified and probed. This enables nanoindentation studies to be carried employing forceversusdistance measurements to measure mechanical properties and in particular, Young's modulus.
For further details of the NanoWizard® and applications from JPK, please visit the JPK web site, www.jpk.com.
JPK Instruments AG
JPK Instruments AG is a world leading manufacturer of nanoanalytic instruments that enable unparalleled access at the nanotechnology level. JPK was recognized as Germany's fastest growing nanotechnology company in 2007 and 2008 (Deloitte). The product portfolio is based around atomic force microscopes and optical tweezers for a wide range of applications, from soft matter physics to nanooptics, from surface chemistry to cellular and molecular biology. Leadingedge instruments from JPK are used by the most renowned research institutes across the world. Headquartered in Berlin and with operations in Dresden, Cambridge (UK), Singapore and Tokyo, JPK maintains a global network of distributors and support centers and provides on the spot applications and service support to an evergrowing community of researchers.
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