Establish Lung Models With the µ-Slide Membrane ibiPore Flow(PresseBox) (Munich, Germany, )
The slide’s horizontal porous glass membrane is inserted between two channels and has brilliant optical quality for high-resolution microscopy. The upper channel above the membrane is a static reservoir. The lower channel is a perfusion channel for applying defined shear stress on cells, which are attached to the membrane. The upper and the lower channel communicate with each other only across the membrane.
To create a lung model with an air-liquid interface (ALI), a polarized cell monolayer (e.g., lung epithelial cells) is cultured on the upper side of the porous membrane, which is exposed to air. From the lower channel, the cells are supplied by culture medium under defined shear stress.
Four different pore sizes are available for various cell types. Researchers can test the slide for their application with a trial pack containing two slides.