siRNA-validated antibodies - Validation data you can trust

Schwerte, (PresseBox) - Look to the new gold standard of antibody validation when you choose your antibodies. Our scientific team is now testing some Thermo Scientific Pierce Antibodies in siRNA-mediated knockdown experiments to ensure specific Western blot detection of target proteins. When you choose one of our siRNA-validated antibodies, you can proceed with even more confidence that you will clearly and unambigously detect the correct protein. See the table below for an example of our Thermo Scientific Pierce Target-Validated Antibodies.

Thermo Scientific Pierce Target-Validated Antibodies accurately detect specific targets. Cells were transfected with Thermo Scientific ON-TARGETplus SMARTpool siRNA Reagents targeting integrin-like kinase 2 (ILK2), STAT1 and AKT2. Cell lysates were analyzed by Western blot. Lane 1: Mock transfection. Lane 2: Control pool siRNA. Lane 3: Gene-specific siRNA. Figure 1: Detection by Supplier A compared with Thermo Scientific Target-Validated ILK2 (52 kDa) Antibody. Specific ILK2 detection and associated knockdown was detected by our validated antibody but not by the corresponding antibody from Supplier A (Figure 1). Figure 2: Detection and knockdown with our AKT2 and STAT1 antibodies.

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