Eurofins MWG Operon Offers Advanced De Novo Genome Sequencing

Use of different types of genomic DNA libraries enables sequencing and scaffolding of genomes of any size

Ebersberg, (PresseBox) - Eurofins MWG Operon, an expert in next generation sequencing (NGS) routinely uses up to 5 different non-cloned genomic DNA libraries for sequencing with Roche GS FLX or Illumina HiSeq 2000 technology. The libraries are a combination of shotgun and long paired end (LPE) libraries with different jumping distances (3 kb, 8 kb, 20 kb and up to 40 kb). After sequencing, the resulting reads are assembled into large contigs and merged into scaffolds. Additional sequencing of normalised or methyl depleted libraries allow deep sequencing of euchromatic regions in highly repetitive genomes.

"Today, the multiple library approach of Eurofins MWG Operon is the most efficient and most economic way to sequence and scaffold genomes", says Prof Ken Wolfe, The Smurfit Institute of Trinity College, Dublin, Ireland. "I was impressed by the excellent quality of the assembly and the fast progress of the project.

"One of the biggest challenges in next generation sequencing is the assembly and scaffolding of the huge amount of sequence data", says Bruno Poddevin, Eurofins Senior Vice President, Genomic Services. "I am extremely pleased with the performance and unique advantages of the LPE libraries which have been developed by our NGS development team. It will allow us to service our customers even more efficiently."

About the Smurfit Institute of Trinity College, Dublin

The Smurfit Institute of Genetics is an academic unit of Trinity College Dublin. The Smurfit Institute and the Moyne Institute of Preventive Medicine together comprise the School of Genetics and Microbiology, which is part of the Faculty of Science. The research and teaching in Genetics encompass and integrate molecular, cellular, developmental, behavioural, medical, psychiatric, population and quantitative genetics and evolution. For further information visit the website at: http://www.tcd.ie/Genetics/index.php

Eurofins Genomics GmbH

Eurofins MWG Operon, a member of the Eurofins Group with major offices and production facilities in Europe, the United States, and Asia, is an international provider of DNA sequencing services, DNA synthesis products and bioinformatic services for academic and industrial research. As one of the first service providers for next generation sequencing, Eurofins MWG Operon has established profound know-how and proprietary protocols to cover a broad range of applications. The company's strength is its strong customer orientation, fast service, and high quality. For further information, please visit our website at: http://www.eurofinsdna.com

Press releases you might also be interested in

Subscribe for news

The subscribtion service of the PresseBox informs you about press information of a certain topic by your choice at a choosen time. Please enter your email address to receive the email with the press releases.

An error occurred!

Thank you! You will receive a confirmation email within a few minutes.


I want to subscribe to the gratis press mail and have read and accepted the conditions.