Carl Zeiss Introduces Lightsheet Z.1 Light Sheet Microscope System
3D fluorescence imaging of large living specimens with low phototoxicity
Biologists can use the new microscopy system to observe the development of entire organisms over several days or more. The extremely low photo- toxicity and the integrated incubation enable in- sights into the differentiation of cell groups without harming the specimen. On large objects, in par- ticular, such as fruit fly or zebrafish embryos, the light sheet microscope delivers more information than established methods of fluorescence mi- croscopy. "The bigger the sample, the more you can get out of it with light sheet microscopy," says Dr. Pavel Tomančák from the Max Planck Institute for Molecular Cell Biology and Genetics in Dresden, Germany, describing the benefits of the new method. Lightsheet Z.1 can also be used in marine and cell biology, as well as plant physiology.
New perspectives with Multiview
Lightsheet Z.1 works with an expanded light beam, the light sheet, that illuminates only a thin section of the sample, thus protecting the rest of the specimen. Images are captured at a 90 degree angle to the light sheet. Therefore, Lightsheet Z.1 achieves maximum image quality at minimal illumination intensity and is particularly well-suited for long-term examinations of living specimens. Multiview imaging allows data acquisition from different viewing angles. These can be combined through mathematical algo- rithms into 3D reconstructions and time-lapse videos.
The light sheet system of Lightsheet Z.1 uses a new type of optical concept that combines cy- lindrical lens optics with laser scanning. Users receive homogeneously illuminated optical sections of complete examination objects.
Dr. Olaf Selchow, Product Manager for Light Sheet Microscopy at Carl Zeiss, states: "I'm confident that this illumination principle is going to revo- lutionize 3D fluorescence imaging."
The Microscopy business group at Carl Zeiss is the world's only manufacturer of both light and electron microscopes. The company's extensive portfolio enables research and routine applications in the life and materials sciences. The product range includes light and laser scanning microscopes, electron and ion microscopes and spectrometer modules. Users are supported for software for system control, image capture and editing. The Microscopy business group has sales companies in 33 countries. Application and service specialists support customers around the globe in demo centers and on site. The business group is headquartered in Jena. Additional production and development sites can be found in Oberkochen, Göttingen and Munich, as well as in the UK and the USA. The company has around 2,500 employees and generates revenue of 620 million euros.
Carl Zeiss AG
The Carl Zeiss Group is an international leader in the fields of optics and optoelectronics. In fiscal year 2010/11 the company's approx. 24,000 employees generated revenue of about 4.237 billion euros. In the markets for Industrial Solutions, Research Solutions, Medical Technology and Consumer Optics, Carl Zeiss has contributed to technological progress all over the world for more than 160 years. With its innovative technologies and leading-edge solutions, Carl Zeiss is successful in the fields of Semiconductor Manufacturing Technology, Industrial Metrology, Microscopy, Medical Technology, Vision Care and Consumer Optics/Optronics. Carl Zeiss AG is fully owned by the Carl Zeiss Stiftung (Carl Zeiss Foundation).