7th Sample Prep 2013 Knowledge Foundation Conference - San Diego CA

(PresseBox) ( Boston, MA, USA, )
- Featuring: "Improved Sample Prep and Target Enrichment for Direct Molecular Detection of Borrelia burgdorferi from Clinical Specimens" by Mark Eshoo, Ibis Biosciences Division of Abbott
- Advance Registration Expires this Friday March 29 -- Register Now & Save $100
- May 9-10 2013 -- San Diego Hilton & Resort, San Diego CA USA

Industry leaders from around the world will convene in San Diego CA, for the Knowledge Foundation's 7th Sample Prep 2013 Conference, to be held at the San Diego Hilton & Resort on May 9-10 2013.

The advanced registration discount expires this Friday March 29, for $100 off your registration fee.


A key presentation will be Mark W. Eshoo PhD, of Ibis Biosciences Inc., An Abbott Company, as he presents, "Improved Sample Prep & Target Enrichment for the Direct Molecular Detection of Borrelia burgdorferi from Clinical Specimens." (see below)

Early detection and treatment of Lyme disease is crucial to prevent late sequelae and to improve long-term prognosis. However, infection is often difficult to diagnose because of the variability of clinical manifestations and the biologically delayed antibody production upon which current serologic tests are based. Direct molecular tests for early Lyme disease have largely been unsuccessful possibly due to the low levels of circulating pathogen.

To address this challenge, we have developed an "ultra-sensitive" molecular assay designed to detect B. burgdorferi directly from whole blood, CSF or other clinical specimens collected as early as the initial patient visit and to detect less than a single genome of Borrelia DNA in a clinical specimen.

Furthermore, this approach also has the potential to detect Borrelia in patients that have already been treated for Lyme and are sero-positive. To achieve our increased sensitivity we employed a larger than typical volume of samples.

Secondly, we performed an isothermal pre-amplification of the Borrelia DNA to increase the copies of Borrelia DNA in the sample.

Thirdly, we detected the enriched Borrelia DNA with a broad-range PCR and electro-spray ionization mass spectrometry assay that targets 8 Borrelia loci each of which is diagnostic for the presence of Borrelia DNA. We compared results of our assay with 2-tiered serology paired specimens collected from 21 endemic-area patients with both clinically and serologically defined early Lyme disease.

Results of this study demonstrated the ability to detect B. burgdorferi in early Lyme disease directly from whole blood specimens prior to seroconversion. This presentation will be given by Mark W. Eshoo PhD, of Biosciences Inc., An Abbott Company, at Sample Prep 2013.


- Robust methodologies for sample collection, (pre-)concentration, lysis, & target extraction
- Critical role of sample prep in early diagnostics
- Rapid sample-to-sequence techniques
- Point-of-care sampling, detection & analysis
- Sample preparation with micro- and nano fluidics
- Robust sampling methodologies (automation, high-throughput, combinatorial approach in sample prep)
- Alternative amplification techniques for sensitive sample prep
- End-user prospective for biodetection & sampling technologies & devices
- Alternative & disruptive approaches to sample preparation & applications
- Field-ready devices: compatibility/reliability/scalability
- Target enrichment & background depletion techniques
- Isolation of dilute objects on cell & molecular levels
- Immiscible phase purification
- Novel methods of extraction from tough (nonfilterable) matrices
- Nucleic acid & protein based sample prep for next generation sequencing
- Nanotechnology & miniaturization challenges for sample preparation
- Standardization & regulatory issues in sample preparation across different applications


- Richard Allen PhD -- Luminex Corp
- Gregory Auner PhD -- Wayne State University
- Patrick Broyer PhD -- BioMérieux SA France
- John C. Carrano PhD Paratus Diagnostics LLC
- Mark W. Eshoo PhD -- Ibis Biosciences Inc Div of Abbott Co
- Sarah Fakih PhD -- QIAGEN GmbH Germany
- Shan Gao PhD -- Wave 80 Biosciences Inc
- Michael J. Heller PhD -- University of California San Diego
- Bruce Irvine -- Claremont BioSolutons
- Hafsa Korri-Youssoufi PhD -- University of Paris-Sud France
- Vasco Liberal PhD -- Biomatrica Inc
- Martin McDonnell PhD -- Defense Science & Technology Lab - DSTL Porton Down UK
- Andy Page -- InnovaPrep LLC
- Forest L. Rohwer PhD -- San Diego State University
- Alexis Sauer-Budge PhD -- Fraunhofer USA
- Marek Smieja MD PhD -- McMaster University Canada
- Numrin Thaitrong PhD -- National Center for Genetic Engineering & Biotech/Sandia National Labs
- Mark Tondra PhD -- Diagnostic Biosensors
- Season Wong PhD -- AI Biosciences Inc.
- David Wright Wi -- Medical Device Development Inc
- Charles Young PhD -- Johns Hopkins University
- David Wright Wi -- Medical Device Development Inc
- Charles Young PhD -- Johns Hopkins University


The Knowledge Foundation integrates scientific ingenuity and real-world applications by fostering collaborative research at the rapidly changing intersection of science and business. Known for providing members of its Technology Commercialization Alliance with direct and unbiased state-of-the-art scientific information, the Knowledge Foundation is uniquely qualified to provide wider exposure for important developments in the emerging fields of alternative energy, nanotechnology, and chemical & biological detection technologies.
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